dnaG [2017-03-31 14:40:40]

DNA primase, part of the replisome

locus: BSU25210

pI: 6.71

mw: 68.62 kDa

protein length: 603 aa

gene length: 1809 bp

function: DNA replication

product: DNA primase

essential: yes

ec: 2.7.7.-

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

This gene is a member of the following [SW|regulons]

• [SW|SigH regulon], [SW|Spo0A regulon]

Gene

Coordinates: 2,601,528 -> 2,603,339

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• required for bacteriophage SPP1 replication [Pubmed|23268446]

Protein family

• DNA primase family (according to Swiss-Prot)

[SW|Domains]

• [SW|TOPRIM domain] (aa 262-343) [Pubmed|9722641]

Effectors of protein activity

• the enzymatic activity is inhibited by (p)ppGpp during the 'stringent response' [Pubmed|17350574]
• [protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] primase activity is stimulates by [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC] [Pubmed|23563155]

Structure

• [PDB|1D0Q] (zinc binding domain, Geobacillus stearothermophilus)
• [PDB|1Z8S] (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
• [PDB|4M4W] (the [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC]-[protein|AC1B507C6B1640E9BEA1F9F01628120F3680C976|DnaI]-[protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] complex) [Pubmed|24048025]

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]-[gene|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG]-[gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
  description: [Pubmed|3298998]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3298998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3127379], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response

  additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]

References

Reviews

• 22797751,17350566,27149325,28075389

Original publications

• 3919021,20122408,14651647,16479537,19415803,17350574,23563155,23268446,21419346,24048025,9849877,9722641